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comA [2017-12-15 17:11:12]
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comA [2017-12-15 17:11:12]

two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)
locus
BSU31680
pI
4.76
mw
23.98 kDa
protein length
214 aa Sequence Blast
gene length
642 bp Sequence Blast
function
regulation of genetic competence and quorum sensing
product
two-component system response regulator
essential
no
ec
4.4.1.19
synonyms
srfB, comAA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    3,252,804 → 3,253,448

    The protein

    Protein family

  • ComA family (according to Swiss-Prot)

    • Modification

  • phosphorylated by [protein|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|ComP] on an Asp residue

    • Effectors of protein activity

  • phosphorylation activates DNA binding
  • DNA-binding activity of ComA is inhibited by [protein|242632B27E0E3AB15777E9FBA3FC746D00CE97F9|RapC] [Pubmed|12950917], [protein|4670F7C4CB9084A5BA478CAE238FA0F8C39F3A7E|RapD] [Pubmed|17227471], [protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF] [Pubmed|15968044,22215984], [protein|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|RapH] [Pubmed|17581123] and [protein|C8BF3815578293542D14811C60F4CA78AACF73EB|RapK] [Pubmed|16816200]

    • Structure

  • [PDB|1U83]
  • [PDB|3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] with [protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF]) [Pubmed|22215984]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|FDACEF9DDC3BEA4EB013AE67BC47ADA532F00205|comQ]-[gene|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|comX]-[gene|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|comP]-[gene|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]-[gene|015989AAB862E418C944AB35DA3605863F059CE9|yuxO]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Biological materials

    Mutant

  • BKE31680 (Δ[gene|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC, downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT
  • BKK31680 (Δ[gene|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC, downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT

    • References

    Reviews

  • 20030732,12576575

    • Original Publications

  • 17227471,11133937,2116363,12067344,8387999,19202088,10094672,8643670,11267663,15968044,16816200,10447896,12950917,20302877,12270822,17581123,2507523,20208433,22215984,24130811,24425772,24788106,25598361,25757097,26582911,26787913,26927849,28033323